![]() ![]() Provide details: Be thorough in outlining the question(s) that you are trying to answer.People from the future may be stuck trying to answer the same question. Report spam or content that is hateful or off-topic.Upvote those who contribute to the discussion and provide freely of their time to assist you.Projects: Share a Link to your pet image analysis project.Research: Links to published (articles in scientific journals or in established repositories) that utilize ImageJ/FIJI for image analysis or are about image analysis.Discussions: Text posts, meant to ask about general issues relating to image analysis.Image analyst job posts are also welcome. Tips: Text or Link posts to share useful how-to tricks and discoveries on using ImageJ/FIJI.Questions which have been Solved will be marked as such. This could include algorithms, microscopy and scientific imaging, plug-ins, methods, and specific features of the software. Questions: Text posts asking about image analysis and ImageJ/FIJI.This is a an unofficial¹ forum to discuss image analysis, software features, to get help, to share ideas, and to share work done using ImageJ or FIJI. It's used worldwide, by a broad range of scientists. Please have a look at the Fiji/Jython script batch-processing example that runs stardist on all files of a folder.ImageJ is a freely available, open source image processing and analysis program using Java, on which FIJI is based. Load default NMS parameters for the selected built-in model.Įxample of running the plugin, showing the returned label image and ROIs overlaid on the input image (check Show All in the ROI Manager):.Increase the number of tiles (in case of GPU memory limitations/errors, i.e.Specify a user-trained model file or URL.The segmented objects can be returned as a Label Image or in the ROI Manager (or both). If in doubt, load the default NMS parameters of the selected built-in model (see below). Overlap Threshold - higher values allow segmented objects to overlap substantially.Probability/Score Threshold - higher values lead to fewer segmented objects, but will likely avoid false positives.If necessary, one can change/disable the percentile-based input image normalization.Īdjust the NMS (non-maximum suppression) postprocessing parameters: A custom user-trained model ( via the training code) that has been exported as a zip file and can be loaded from a file or URL (see Advanced options below).Versatile (H&E nuclei) that was trained on images from the MoNuSeg 2018 training data and the TCGA archive.Versatile (fluorescent nuclei) and DSB 2018 (from StarDist 2D paper) that were both trained on a subset of the DSB 2018 nuclei segmentation challenge dataset.Select a neural network model from the dropdown list, which can be one of the following: Start the plugin from Plugins > StarDist > StarDist 2D. Suitable test images can for instance be found at the Broad Bioimage Benchmark Collection: Note, that currently only 2D and 2D+time images are supported. Click on Apply changes to install the plugin.(If StarDist is missing, click Update URLs to refresh the list of update sites.) Scroll down the list and tick the checkboxes for update sites CSBDeep and StarDist, then click the Close button.Click on the button Manage update sites.Start Fiji (or download and install it from here first).If you have 3D data, please use our python library. The plugin currently only supports 2D image and time lapse data. ![]() Please only file an issue here for bug reports or if you have technical questions regarding the plugin. If you open a new topic, please provide a clear and concise description to understand and ideally reproduce the issue you’re having. If you can’t find what you’re looking for, please create a new topic at the forum (and use the tag stardist). If that doesn’t solve your issue, you can browse the existing stardist-tagged forum posts or search the image.sc forum. ![]() If you need any help, please first take a look at the StarDist documentation and frequently asked questions (FAQ). See the main repository for links to our publications and the full-featured Python package that can also be used to train new models. The plugin can be used to apply already trained models to new images. This is the ImageJ/Fiji plugin for StarDist, a cell/nuclei detection method for microscopy images with star-convex shape priors. If you’d like to help, check out the how to help guide! The content of this page has not been vetted since shifting away from MediaWiki. ![]()
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